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Abstract Hydrogen peroxide (H₂O₂) mediates the biology of wound healing, apoptosis, inflammation, etc. H₂O₂has been fluorometrically imaged with protein‐ or small‐molecule‐based probes. However, only protein‐based probes have afforded temporal insights within seconds. Small‐molecule‐based electrophilic probes for H₂O₂require many minutes for a sufficient response in biological systems. Here, we report a fluorogenic probe that selectively undergoes a [2,3]‐sigmatropic rearrangement (seleno‐Mislow‐Evans rearrangement) with H₂O₂, followed by acetal hydrolysis, to produce a green fluorescent molecule in seconds. Unlike other electrophilic probes, the current probe acts as a nucleophile. The fast kinetics enabled real‐time imaging of H₂O₂produced in endothelial cells in 8 seconds (much earlier than previously shown) and H₂O₂in a zebrafish wound healing model. This work may provide a platform for endogenous H₂O₂detection in real time with chemical probes.